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However, more human studies are needed to confirm whether supplementing with B5 might improve adrenal function

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Afterwards, the fluorescence was developed with a 0.5 mg/mL trypsin solution (final concentration), and the fluorescence readout was done with an Envision 2104 Multilabel Plate Reader (PerkinElmer, Waltham, USA) with λ Ex = 380 ± 10 nm and λ Em = 430 ± 8 nm