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A6 cells were added to 96 well plates at 2.5 x10 4 cells per well, incubated at 27°C overnight, then exposed to 10 mM BSO or 2.5 mM cysteine for 18 hours, before determining cell viability using CyQUANT™ MTT assay following the manufacturer’s instructions
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Dysregulated autophagy mediates sarcopenic obesity and its complications via AMPK and PGC1α signaling pathways: potential involvement of gut dysbiosis as a pathological link
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