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The mixture was heated to 65 °C for 5 min and quickly chilled on ice

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A typical research peptide journey: Synthesis in a Chinese facility that may or may not follow Good Manufacturing Practices (most don’t, because research chemicals are not classified as pharmaceuticals in China) Export through intermediary trading companies that handle customs documentation Import by a US-based vendor who may or may not perform incoming quality testing Labeling and packaging by the vendor, who assigns a lot number and may or may not conduct identity and purity testing Sale to a consumer who reconstitutes, doses, and injects the product At each step, there are opportunities for contamination, degradation, substitution, and fraud

resolution purification of tagged proteins