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2.4 Library preparation and genotyping by sequencing Seeds of 132 genotypes of buckwheat were sown in plastic trays for 3 weeks in a polyhouse and the harvested shoots were used for genomic DNA extraction using the CTAB method ( 2.5 Post sequencing analysis The raw reads were filtered for adapter sequences, low-quality reads, and low-quality residues towards the 5′ region of the sequence
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