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A total of 1 × 10 9 frozen cells (8 × 10 8 Expi293, 5 × 10 7 K562, 1 × 10 8 U937 and 5 × 10 7 Hep3B cells) were resuspended in 20 ml lysis buffer (50 mM Tris pH 8.0, 200 mM NaCl, 2 mM TCEP, 0.1% NP-40, 2 µl Benzonase (EMD Millipore 77664-3), and 1 tablet of cOmplete protease inhibitor cocktail)
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