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Inhibitory Effect of Blueberry Polyphenolic Compounds on Oleic Acid-Induced Hepatic Steatosis in Vitro

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Cells were washed twice with the FLICA kit wash buffer and then incubated with LIVE/DEAD Fixable AQUA Dead Cells Stain (Thermo Fisher, USA) for 15 min at RT, followed by extracellular staining in PBS + 1% BSA with the following fluorochrome-conjugated antibodies for monocyte phenotyping: anti-CD14 BV605 (Clone:M5E2), anti-CD16 PE-Cy7 (Clone:3G8), and anti-CD3 PE (Clone : HIT3a) from BioLegend, San Diego, CA, USA