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Based on the results of this assay, sample aliquots were adjusted to 10 µg protein per 10 µL and mixed with Laemmli sample buffer (with 25% glycerol (Carl Roth, Karlsruhe, Germany), 5% sodium dodecyl sulfate, 5% β-mercaptoethanol (Sigma-Aldrich Chemie, Steinheim, Germany), 150 mM Tris pH 6.8, 0.05% bromophenol blue (Merck KGaA, Darmstadt, Germany)
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