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Whole-cell lysates were prepared using NaCl Lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium phosphate, 1 mM ß-glycerophosphate) containing a protease inhibitor cocktail (Roche/Sigma) and phosphatase inhibitor (Thermo Fisher) following standard methods

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Meanwhile, we also obtained PS-COOH according to the previous literature 52