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Kerry Campbell, was cultured in alpha-MEM containing ribo- and deoxyribonucleosides, supplemented with 12.5% heat inactivated (HI) horse serum (Sigma-Aldrich), 12.5% HI fetal calf serum (FCS), 2.2 g/L sodium bicarbonate, 0.2 mM inositol (Sigma-Aldrich), 2 mM L-glutamine, 0.1 mM 2-mercaptoethanol (Life Technologies), 0.02 mM folic acid (Sigma-Aldrich), and 200 U/mL recombinant IL-2 (Chiron)