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Alopecia Areata: Pathogenesis, Diagnosis, and Therapies - PMC

For experiments, cells were cultured on round coverslips in 12-well plates and then stabilized for 30 min with Dulbecco's modified Eagle's medium supplemented with 5 mg/mL apotransferrin, 1 mg/mL bovine serum albumin, 25 mM HEPES (pH 7.4), 2 mM glutamine, 100 units/mL penicillin, and 100 mg/mL streptomycin (serum-free medium)

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However, treatment with antibiotics or non-antibiotic drugs could also induce dysbiosis and may induce the acquisition of antimicrobial resistance, affecting the pharmacokinetics of therapeutical drugs (Maier et al., 2018)