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the membrane potential was held at -70 mV at room temperature (25°C) during a period of 0.5 - 2 h after removal of the culture from the incubator
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Briefly, coronal brain sections were immersed in a 1:9 (v/v) solution of 1% sodium hydroxide and 70% ethanol for five minutes, followed by a two-minute wash in 70% ethanol
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