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Hypothyroidism is usually treated using levothyroxine, a synthetic form of the thyroid hormone thyroxine (T4), to replace the deficient thyroid hormones
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Thus, to compare the activity of the wild-type and TAT-tagged TrxR1 enzymes, we measured the DTNB reduction activity of TrxR1 and TAT-TrxR1 at 50 nM and 100 nM concentrations over a time course (Figure 2B)
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