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The xenograft tumors were intratumorally injected with shScramble, shUSP33#1, shUSP33#2, shUSP33#1 + Flag-SIN1 or shUSP33#1 + Flag-USP33 WT, shUSP33#1 + Flag-USP33 2A lentivirus at a dose of 1 × 10 8 pfu/100 μL per mouse every 3 days for three times to knockdown or overexpress USP33 expression in the tumor [16,17,18]
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