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Briefly, cell pellets were resuspended in 30 ml phosphate buffer (100 mM potassium phosphate (PB0445, Biobasic), pH 7.2, 10% glycerol (CA97063-892, VWR)) supplemented with lysozyme (1 mg/ ml) (12650-88-3, Biobasic) and disrupted by sonication
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Hepcidin is down-regulated in alcoholic liver injury: implications for the pathogenesis of alcoholic liver disease
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