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SPOC proteins regulate pause release, mRNA stability, and m6A abundance To probe the cellular function of the SPOC domain in the context of gene expression regulation, we generated DIDO KO and DIDO ΔSPOC, RBM15 and RBM15 ΔSPOC, as well as SHARP ΔSPOC-GFP HEK293T cell lines using CRISPR/Cas9 (Supplementary Figs

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PROPKA3: consistent treatment of internal and surface residues in empirical pK predictions