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For each FoV, MitoTracker Red CMXRos was used to detect and calculate the total active mitochondria area (by using the ‘Filter and Find Image’ and ‘calculate morphology and properties’ building blocks)

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Moreover, HMOX1/HO-1 (heme oxygenase 1) was reported to promote lung metastasis in mice, and its high expression was correlated with tumor invasiveness in NSCLC, as similarly reported for HIF-1α [30,31,32]