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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins
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Cell tracking with 5-ethynyl-2′-deoxyuridine (EdU) and confocal live imaging showed that in epithelioids, as in vivo, some cells exit the basal layer, migrate through the suprabasal layers and are eventually shed (Fig
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