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The slides were transferred to a container with basic incubation buffer (20 mM KCl, 2.7 mM ATP, disodium salt, 50 mM CaCl 2 dihydrate, 100 mM 2-amino-2-methyl-propanol HCl [pH 10.5], with 1 N HCl, not filtered) and incubated at 24 °C for 25 min
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Cantó, C., Menzies, K., & Auwerx, J
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