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Antioxidant effects after coffee enema or oral coffee consumption in healthy Thai male volunteers - S Teekachunhatean, N Tosri, C Sangdee, R Wongpoomchai, W Ruangyuttikarn, C Puaninta, S Srichairatanakool, 2012

PVDF membranes were blocked in 5% (w/v) milk powder in PBST (140 mM NaCl, 2.7 mM KCl, 10.1 mM Na 2 HPO4, 1.8 mM KH 2 PO4, 0.1% (v/v) Tween-20) or TBST (150 mM NaCl, 20 mM Tris/HCl, pH 7.5, 0.1 % (v/v) Tween-20) for 1 h at room temperature

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Interestingly, at the end of a three-month follow-up, although both groups showed significant improvements in pain assessed by VAS, the change in the B12 arm was significantly greater relative to the nortriptyline group