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NAD+ is indispensable for energy metabolism — glycolysis, the citric-acid cycle, fatty-acid oxidation — and it is the substrate that sirtuins and PARPs consume

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For flow cytometry, treated cells from 24-well plates were washed twice with Hank’s Balanced Salt Solution (HBSS), incubated with 50 nM TMRE for 30 min at 37 °C in the dark, washed twice with HBSS, trypsinized, and resuspended in HBSS supplemented with 10% FBS