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How to Calculate Your Injectable GHK-Cu Dose Step 1: Determine Your Vial Concentration After reconstitution, concentration depends on vial size and volume of bacteriostatic water added: Step 2: Calculate Units on Your Syringe Using a standard U-100 insulin syringe (100 units = 1 ml): Formula: Units to draw = (Desired dose in mg ÷ Concentration in mg/ml) × 100 Example calculations at 2 mg/ml concentration: Example calculations at 1 mg/ml concentration: Quick Reference Card 10 mg vial reconstituted with 5 ml (2 mg/ml) Reconstitution: Getting It Right What You Need GHK-Cu lyophilized powder vial Bacteriostatic water (not sterile water or saline) Sterile syringes, one for mixing, separate ones for injections Alcohol swabs Clean, flat work surface Refrigerator for immediate storage post-reconstitution Reconstitution Process 1

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In rats the TISSUE half-life is measured in days rather than hours: roughly 12.7 days after intravenous dosing and 8.8 days intraperitoneally, with accumulation in brain