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CONCLUSIONS The assay is simple and faster than can be performed even on a semiautomatic colorimeter, so the present protocol is suitable for routine assays, particularly because it is suggested to measure both enzymes with the same substrate (ATCh), color reagent, and buffer solutions
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The transcription factors related to DN mainly include HIF1α, KLF4, KLF5, RUNX1, SP1, VDR, and WT1
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