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After that, the cells were blocked with phosphate-buffered saline (PBS) containing 5% bovine serum albumin (BSA) for 1 h and then incubated with anti-HSV-1 gD antibody (1:1,000 in 5% BSA) overnight, followed by staining with FITC-labeled goat anti-mouse IgG (ABclonal) (1:1,000 in 5% BSA)
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