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In practical terms, a 99% HPLC purity figure means that 99% of the UV-absorbing material the detector saw corresponds to the target peptide, and the remaining 1% is peptide-related impurities such as deletion sequences from incomplete synthesis, oxidation products, or dimers
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To verify FBXL5 deletion and its subsequent effect on cellular iron levels in CD11c + myeloid cells, we isolated CD11c + cells from the spleens of Cd11c -Cre/ Fbxl5 F/F (hereafter referred to as FBXL5 ΞCD11c ) and Fbxl5 F/F (control) mice
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