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15LO1 increases intracellular oxidative potential by lowering GSH and increasing extracellular GSSG To confirm that 15LO1-PEBP1 complex activity alters IL-13–induced intracellular GSH levels, we modeled these relationships in vitro by stimulating cultured HAECs (at ALI) with IL-13 for 7–10 days in the presence and absence of 15LO1 protein (dicer siRNA knockdown [KD] of ALOX15 gene) or enzymatic activity (specific 15LO1 inhibitor BLX2477) (14, 15)

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In thermal-oxidation (i.e., radical oxidation), all isomers of both PC 16:0/22:6;OOH and TG 18:1_18:1_22:6;OOH tended to decrease throughout the oxidation period corroborating the instability of DHA;OOH 34 (Fig